Place this clip early in a horror sequence to set the scene, then follow it with the FNAF jumpscare scream for the reveal, before moving on to your next gameplay beat without adding another effect underneath it.
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This clip is a 34-second audio file labelled A-120, presented on its own as a single extended sound rather than a short hit. It carries a tense, uneasy feeling that builds unease while it plays, making a listener wait for something to happen instead of giving an instant payoff or a quick laugh.