Place it at the end of a repeated gag or argument, after the setup has run its course, so it closes the scene instead of starting one. Pair it with Sad Violin for a mock-tragic exit, or follow it with Nelson's Ha-Ha! when the other person keeps going anyway.
このサウンドについて
This is an eight-second Portuguese voice clip sighing 'Ai meu Deus do céu... Já deu' in a weary, fed-up tone, stretching the first phrase before cutting it off with a blunt dismissal. It feels exasperated and done, like the last word in an argument that has gone on too long, and it lands best as a short, dramatic shut-down.