Place it right after the loudest moment in your edit, then follow it with a short reaction such as the Bruh sound to underline the pause, or open with the Game Show Buzzer sound before it to signal that the chaos has to stop.
このサウンドについて
This short clip repeats the Portuguese phrase calma cara, calma cara, calma in a firm, insistent voice for about three and a half seconds. It feels like a playful but exasperated interruption, the kind of line that cuts through noise and tells everyone to pause. It was uploaded as a way to get people to stop what they are doing, inspired by an idea from @corinthiana.