Place it early in a horror edit while the scene builds toward a reveal, then cut to the FNAF Jumpscare Scream at the reveal for the payoff. For a lighter scare, follow it with the Danger Alarm Meme Sound instead, keeping Freddy's tune as the opening cue.
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This 17-second clip plays Freddy Fazbear's delicate, tinkling music box melody from Five Nights at Freddy's, a tune that begins almost playful before turning uneasy. It creates a creeping sense of dread, like waiting in the dark for something to move just out of sight, and fans instantly recognise it as the signal that Freddy is near.