The sound comes from Lonesome Ghosts, a 1937 Mickey Mouse cartoon in which Mickey, Donald Duck and Goofy take a job removing ghosts from a haunted mansion, only to be tormented by the spirits themselves. The screaming and laughing heard here belongs to those prankster ghosts as they enjoy frightening their would-be exterminators. Decades later the clip found a second life on soundboards and in short-form videos, where its vintage cartoon tone makes it instantly recognisable. It remains popular because it sounds spooky without being genuinely scary, so it suits comedy as well as Halloween content.