Place it at the turning point of a clip, just as someone commits to a stunt or challenge, then follow it with Victory Sound Effect once the attempt lands. For a bigger build-up, open with Leeroy Jenkins before it so the charge feels bigger.
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This is a short, loud shout of 'Just Do It' delivered by Shia LaBeouf in a forceful, urgent voice, lasting about a second and a half. It feels like a sudden jolt of encouragement, funny because of how intense it is and motivating because it leaves no room for hesitation. The line comes from his well-known motivational speech performance.