Place it in the middle of a short edit, just after the frog is shown and before the rescue or safe landing, then follow it with Vine Boom for a sharp contrast or Anime Wow when the frog suddenly appears safe.
このサウンドについて
This is a soft, restrained scream lasting about thirteen seconds, voiced in a hushed, worried tone as if someone has just spotted a small frog in trouble. It sounds gentle rather than panicked, more like a held-back gasp stretched into a cry, and it gives listeners a tender, slightly anxious feeling, mixing concern for the tiny creature with a quiet kind of humour.