Place it at the end of a short exchange about being ignored, after the unanswered message has been shown on screen. Follow it with a laugh for contrast, or open with a duo reference before the abandonment lands so the joke has a clear target.
このサウンドについて
This is a very short Portuguese voice clip built around the phrase 'me abandonou', lasting about a second and a half. The voice delivers the line with an exaggerated, heartbroken tone that makes being left behind sound both dramatic and funny, turning a small moment of rejection into a theatrical complaint that invites sympathy and a laugh at the same time.