Place it at the peak of a failed attempt, right after the mistake becomes clear to everyone watching. Follow it with the No No Wait Wait Sound Button when the situation gets worse, or end the sequence there if the next scene already answers the call.
このサウンドについて
This is a short voice clip of someone stretching out a loud call of 'Mooooooom' in one urgent burst. It sounds needy, dramatic and a little childish, like a plea that cannot wait another second. That over-the-top desperation is what makes listeners laugh, because everyone recognises the tone of calling for help over something tiny.