Put it at the end of a normal Mario sequence, after Mario Jump sets up the familiar game moment, then let Vine Boom underline the reveal for anyone watching the edit.
このサウンドについて
This is a brief 1.7-second jumpscare clip built around the Nightmare Mario.exe head, a distorted horror version of Mario that appears without warning. The sudden burst makes it feel startling and unsettling, like a scare that arrives before you have time to react, and it works best as a quick shock rather than a sound to sit with.