Place it at the exact moment of the mistake in your edit, then follow it with the mission failed sound button for a bigger fail or the ba dum tss sound button if you want it read as a joke instead of a disaster.
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This is a roughly nine-second clip built around a single confusing noise that could pass for a computer error alert or an embarrassing fart. It never settles the question, which is the point. The feeling is pure awkward comedy, gross and silly at once, perfect for that split second when something has clearly gone wrong.