Place it after the reveal in the middle of the edit, then follow it with Emotional Damage for a second reaction beat before closing with Bruh as the final tag.
このサウンドについて
This is the Sad Vierra clip, a roughly 37-second meme sound centred on a sad, downcast mood. Its length gives the disappointment room to land, and it gives a listener a heavy, sorrowful feeling that makes an awkward or unlucky moment feel larger and more theatrical. It works best as an emotional underline for a failed plan or a quiet defeat, rather than as background noise.