Place it at the exact frame of the bad reveal, then follow with the eww brother eww reaction for a second disgusted beat. If the moment needs a bigger payoff afterwards, layer in a damn meme hit last so the sequence escalates instead of repeating the same joke.
このサウンドについて
This is a sub-second reaction clip built around the single blunt word 'Shitfish', delivered as a quick punchy exclamation. At under a second it lands as an instant burst rather than a drawn-out line. It gives a listener a mix of disgust, disbelief and silly shock, like a gross-out punch word you fire off when something looks or smells completely wrong.