Place it right after the teasing setup in your edit, then follow it with the Anime Wow for a surprised reaction and a Vine Boom to underline the stop. Keep it as the centre of a single reaction beat, with the other sounds framing it rather than competing with it.
このサウンドについて
This is an 8-second voice clip of a high-pitched voice pleading 'yamete kudasai' in Japanese, drawn out with dramatic, desperate emphasis. It feels awkward, playful and deliberately over the top, like a cartoonish cry for someone to stop. The exaggerated delivery makes it land as a joke rather than a serious plea.