Spot Holes 2 comes from Daniel Pemberton's original score for Spider-Man: Across the Spider-Verse, the 2023 animated sequel in which the Spot, voiced by Jason Schwartzman, discovers he can open portals between dimensions. The cue underscores his strange, growing power and the film's shifting multiverse, and clips of it spread through fan edits, reaction videos and soundboards after the film's release. It remains popular alongside the Prowler Sound Effect and Miguel O'Hara, because it instantly signals a Spider-Verse villain moment without needing dialogue or a visual reference.