Place it right after the creepy line or suggestive clip, as the direct response that ends the bit, then follow it with Eww Brother Eww for the follow-up reaction, or lead into it with No No Wait Wait when someone is about to cross the line.
このサウンドについて
This is a short voice clip of someone saying 'Stop. Being. A. Pervert!' in a firm, scolding tone, lasting about three and a half seconds with each word given its own beat. It feels awkward and confrontational in a funny way, like catching someone mid-creep and shutting the moment down before it can get worse.