Place it just before the reveal that separates the two things being compared, then follow it with a reaction sound once the difference is clear. Pair it with It Was At This Moment He Knew for the setup and Bruh Meme for the response, keeping the comparison itself as the centre of the edit.
このサウンドについて
This is an eight-second spoken line built around the phrase 'there is a big difference between', delivered in a measured, declarative voice that leaves the comparison hanging for the listener to finish. It feels pointed and slightly smug, like a calm correction dropped into an argument, and works because the unfinished comparison invites someone to supply the punchline themselves.