Place this clip right after the on-screen request in your edit, then follow it with the No No Wait Wait button when the character tries to backtrack, or precede it with a wrong answer buzzer for a quiz-style refusal sequence that ends the scene.
このサウンドについて
This is a 3.7-second voice clip built around the short spoken phrase 'utha no,' said plainly as a blunt refusal. It feels dismissively funny rather than angry, like a deadpan shutdown that turns a small rejection into a joke and leaves the other person with nowhere to go next in the argument.