The line comes from South Park, the long-running animated series centred on four schoolboys in the fictional Colorado town of South Park, with Kyle as one of its main characters. Short voice clips from the show have long circulated on soundboards and in fan edits because their blunt, quotable delivery works without much context. This one has survived as a reaction sound rather than a reference to a single scene, since the question can be aimed at almost anyone caught doing something foolish, suspicious or badly timed.