The line comes from Austin Powers: International Man of Mystery, released in 1997, during a bathroom stall scene where Austin is fighting an attacker while a neighbouring character mistakes the struggle for constipation. Number Two is Dr Evil's deputy, played by Robert Wagner, so the question works as both a plot reference and toilet humour. The clip later circulated on soundboards and in short video edits, where its short length and delayed pun made it easy to drop into unrelated awkward moments, and it remains a familiar quote from the film.
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Austin Powers asks who does Number 2 work for in this short movie quote, a toilet humour line people use as a reaction sound effect in edits and chats.
This is a short voice clip asking who does Number 2 work for, playing on Number Two being Dr Evil's second in command and a slang term for going to the toilet. The delivery is loud, earnest and encouraging, as if it were serious support from the next bathroom stall, which makes the crude double meaning land harder. The joke works because the wording sounds like an interrogation for a few seconds before the listener catches the pun. It leaves viewers amused and caught off guard, making the line instantly quotable out of context.