Place it at the peak of a villain reveal, right after the action freezes. Follow it with a wtf boom for a chaotic exit, or close the clip on a to be continued card when the joke needs a sequel tease.
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This clip is a nearly five-second burst of deep, booming male laughter in the unmistakable Zehahaha style of Blackbeard. It feels gleefully evil and overconfident, like a villain who just got exactly what he wanted and wants everyone to hear it. The laugh comes from the anime character Marshall D. Teach in One Piece.