Place it at the end of a short fail sequence, after the setup and just before the cut to black. Build tension first with Sudden Suspense, follow it with Oh My God Wow, and use Cartoon Slip for the moment that causes the fall.
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This is a single drawn-out human scream, a long 'aaaaahhhhh' that lasts about three and a half seconds. It feels panicked and over the top, funny because it is so dramatic, and suited to a moment that suddenly goes completely wrong.