Dame is a common Japanese word meaning no good, stop, or don't, and it appears constantly in anime dialogue when a character protests or panics. Short clips repeating the phrase became popular on soundboards and in edits alongside other catchphrase sounds like Tuturu and Yamate Kudesai, because two syllables are instantly recognisable and easy to loop. The dame dame version spread as a reaction meme rather than a specific quote, used whenever a creator needs a quick, cute way to say absolutely not without adding dialogue or explanation.