Place it as the final beat when a character dies in your edit, not under earlier action. Build up with a Bruh Meme Sound Button for a minor mistake first, then switch to this clip for the actual game over, and follow it with a Fail Sound Meme Button only if you are cutting to a scoreboard.
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This is the 6-second death effect from Half-Life 2, built around the suit's flatline tone and fading system sounds as the player character dies. It feels bleak and final rather than jumpy, turning a simple game over into a quiet, clinical shutdown that gamers instantly recognise from the series.