The line comes from the Clayton Bigsby sketch on the first season of Chappelle's Show in 2003, where Dave Chappelle plays a blind man who does not know he is Black while leading a white supremacist group. The sketch became one of the show's most quoted early pieces, and short clips of Bigsby's speeches later spread on forums, YouTube and soundboards. This two second excerpt survived because the phrase works out of context as a mock sermon about resentment, so creators reuse it whenever anger looks theatrical or misplaced.
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Dave Chappelle as blind character Clayton Bigsby delivers the hate in your heart line from Chappelle's Show, now used as a sharp reaction sound.
This is Dave Chappelle performing as Clayton Bigsby, the blind Black white supremacist from Chappelle's Show, delivering his line about letting hate out of your heart. The delivery is loud, raspy and completely dead serious, which is what makes the moment so absurd and memorable. In just over two seconds the voice builds from a preachy warning into a shouted punchline, landing like a scolding from someone who has no idea how contradictory he sounds. Online it works as an instant reaction to bitterness, petty grudges or over the top anger in a conversation.