Put it at the start of a scene as the cue that something worth noticing is coming, then follow it with the Metal Gear Alert Sound when the reveal lands. For a lighter payoff, use the Yeah Reaction Sound after the joke instead, keeping the alert first and the reaction second so the moment builds in order.
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This is Navi's quick 'Hey, listen' call from The Legend of Zelda: Ocarina of Time, a bright, high-pitched fairy voice lasting just over a second. It feels cheerful, insistent and a little cheeky, like a companion tugging at your sleeve for attention. The line comes from the 1998 Nintendo 64 game, where Navi repeated it while guiding Link, which is why players still recognise it instantly.