Place it immediately after the reveal of a failed plan, then follow it with the SpongeBob Fail sting to close the joke, or lead in with Dun Dun Dun for tension before it lands and the Vine Boom Sound Button for a heavier punch.
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This short clip is a burst of repeated 'ho ho' laughter, lasting just over two and a half seconds, with a teasing, mischievous edge that makes it feel like someone has just caught you out. It comes from HowToBasic's 'How to defend yourself'.