Place it as the first reaction beat after a reveal, then follow it with a laugh or a shocked follow-up to keep the joke moving. Pair it after Sudden Suspense for a bigger build-up, or before Nelson's Ha-Ha! when the surprise turns into teasing.
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This is a short, just over four-and-a-half-second vocal clip centred on one surprised exclamation, Holy Schmoly. It gives a feeling of playful disbelief and amused shock, the reaction you reach for when something unexpected is too silly to take seriously, and it leaves the moment feeling lighter and bigger than it first seemed.