Put this clip at the reveal in an anime scene, then follow it with JoJo To Be Continued to freeze the moment as a cliffhanger. For a bigger build-up, place Za Warudo Time Stop just before it, so the freeze sets up the reaction that follows.
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This is a very short startled vocal reaction from Koichi, lasting just over half a second. It feels sudden, nervous and funny, like a jump scare caught in the moment before he can recover, and it works best as a tiny burst of panic rather than a longer line.