Place it immediately after the bad idea appears on screen, then follow it with the Dun Dun Dun sting to underline the rejection. For a harsher cut, pair it with the Classic Error Sound instead, keeping the refusal as the final word in the sequence.
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The clip is a scientist from Half-Life 2 delivering a flat, single-word refusal, 'No', in just over a second. It feels blunt, weary and instantly dismissive, like a firm answer shutting down a bad suggestion before it goes any further. The line comes from Valve's Half-Life 2, where the scientist's deadpan delivery makes the brief denial funnier than a longer argument would.