The line comes from Atop the Fourth Wall, the long-running web series in which reviewer Linkara critiques comic books panel by panel. Whenever a comic leans on dubious comic-book science, he breaks character to deliver this theatrically exasperated retort, and the moment became a recurring catchphrase. Fans clipped the delivery and passed it around forums and video edits, where it outgrew its comic-review roots. Today it circulates as a standalone reaction sound, used whenever someone states the obvious with total confidence or asks a question science answered long ago.
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Linkara from Atop the Fourth Wall asks if you know anything about science. Meme makers use it to mock obvious questions or punctuate over-the-top explanations.
The clip captures Linkara, host of the web series Atop the Fourth Wall, delivering his incredulous catchphrase in a theatrically exasperated tone: of course, don't you know anything about science. His voice rises with mock disbelief, stretching the words just enough to land the joke without tipping into shouting. It sounds like a teacher who has explained the same obvious fact one too many times, equal parts smug and fed up. Dropped into an edit, it instantly frames whatever came before as hilariously self-evident, making the viewer feel in on the joke while the target of the gag looks clueless.
