Put it right after the reveal in a reaction edit, not under the build-up. Start the sequence with a short huh sound to set up the confusion, follow with this clip as the main reaction, then close with an oh my god wow sound if the moment needs a second, bigger payoff from the crowd.
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The clip is a stretched-out voice exclamation in Portuguese, dragging out 'Nossa' before landing on 'que viagem' over about seven seconds. It sounds like pure stunned disbelief, the kind of slow, spaced-out reaction you give when something is too weird to process right away. It is funny because the delivery keeps building long after a normal reaction would end.