Place it at the end of a scene, after the twist is revealed, as the final beat before a cut to black. Build tension first with the Inception Horn, then follow the laugh with the FNAF Jumpscare Scream for a sharp scare that closes the sequence.
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This clip is a theatrical, maniacal villain's laugh, lasting just over five seconds of cackling. It feels gleefully sinister and unsettling, like a bad guy savouring a cruel joke at someone else's expense. It comes from Henry Stauf, the toymaker villain of the classic puzzle adventure game The 7th Guest.