Put it near the end of a scare sequence, after Dun Dun Dun builds the tension and just before the Vine Boom hits for the reveal, so the laugh becomes the payoff rather than the setup.
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This is a very short laugh, just a quick burst of uneasy laughter that barely lasts a blink. It feels tense and unsettling rather than joyful, like someone laughing at something they should not find funny, which makes it perfect for a sudden scare. The clip comes from the horror game Welcome to the Game 2.