Place this clip at the very end of a fail sequence as the closing verdict, after the mistake is already clear. Follow a Lego Yoda death sound with it for cartoon losses, or lead into the Fatality sound when a game finish needs a bigger payoff.
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A short cartoon voice line delivers the words we're all dead in about three seconds, stated flatly as a final verdict. It feels funny and hopeless at once, like panic that has already given up and accepted the worst. The clip comes from Aqua Teen Hunger Force.