Put it at the very start of the sequence, just before the action begins, then follow the go with Okay Let's Go for a spoken launch or the Run Vine sound when a chase or sprint takes over. Keep it as the opening cue and let the next clip carry the action.
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A spoken countdown runs for eight seconds, with three, two and one arriving in quick succession before a bold go closes it. It feels tense and exciting, like waiting for a start when everyone is ready to move. It comes from the familiar race and game starting signal used to give everyone the same moment to begin.