Place it at the end of a failed joke or awkward reveal, then follow it with Emotional Damage for a bigger reaction or a Game Show Buzzer to close the bit. For a joke run, put Fart With Reverb just before it and let this clip deliver the final beat.
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In about two seconds, a quick fart sound gives way to a drawn-out, theatrical moan that arrives right behind it. The jump from crude to melodramatic feels absurd and awkward, catching listeners off guard and turning disgust into a laugh. This version is credited to an upload by Mango.