Place it at the end of a reveal, after a setup beat, so the turn lands as the punchline. Pair it after the Shocked Meme Sound Effect for a sudden reveal, or before the Oh No No No Laugh when the joke keeps getting stranger.
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This is a short, distinctive laugh linked to Image_Friend in Deltarune Chapter 3/4, lasting just under five seconds. It sounds bright and playful at first, then turns oddly uncanny, leaving a listener amused and slightly uneasy at once. That cheerful yet unsettling mix makes it easy to recognise for fans of the game.