In an edit, it belongs immediately after the cut to the unexpected image, as the opening reaction in the sequence, with the Woody Woodpecker Laugh following it. For a longer build, put You What first, let that reaction play out, and then use this clip as the second reaction.
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This three-second voice clip is a single spoken exclamation, “Oh my glob!”, delivered as a quick cartoon reaction. It gives a listener an instant sense of wide-eyed disbelief mixed with playful shock, turning an odd or unexpected reveal into something funny rather than serious. The line comes from Adventure Time, where characters use it as a stand-in exclamation in moments of surprise.