Place it at the climax of a scare sequence, after Sudden Suspense and just before the reveal shot, then follow it with Oh My God Wow for the shocked reaction that closes the moment.
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This brief scare effect lasts about 3.9 seconds, hitting fast with a sharp burst of dread and then sustaining that nervous tension long enough for a reveal or a wide-eyed reaction to land. It leaves listeners startled and uneasy, making a jump scare hit harder and a simple prank feel far more alarming than it looks.