The exact recording behind this clip is not tied to one named film or series, but the style comes from decades of comics and cartoons where a single exaggerated neigh stood in for any horse on screen. Sound editors reused that kind of whinny for westerns, circus scenes and slapstick, and it later spread online as a standalone effect for edits and soundboards. It remains popular because one cartoon chase or farm scene needs no setup to be understood, and the neigh reads clearly even on a phone speaker.