Place it at the end of a short refusal scene, after the request has been shown, then follow it with Anime Ahh for a quick reaction or Awkward Moment to let the rejection hang before the next scene starts.
O tym dźwięku
This short clip, just under four seconds, has Hatsune Miku delivering a drawn-out refusal in her clear, high-pitched Vocaloid voice. It feels playful and stubborn at once, like a cute but firm shutdown that still makes people smile. It comes from Bunchi's version of the 'Anon Noooo' meme, revoiced with Miku herself instead of the original speaker.