The line comes from Monster Factory, the McElroy brothers' long-running series in which they build exaggerated characters in video game creators and play through the results. Moments of slapstick pain and over-the-top reactions became a natural source of short reaction clips, and this brief groan was picked up for soundboards because it is instantly understandable without context. It remains popular in gaming edits and comedy videos, where a quick, pained reaction can sell a failed jump, a bad hit or an awkward fall.