The clip is tied by its original file metadata to a Steam Train playthrough of Undertale's genocide route, titled Nothing Lives! Part 1, in which Ross laughs during the run. Undertale, the indie role-playing game by Toby Fox, became known for that darker route, where players deliberately defeat every monster, and moments from those playthroughs were widely clipped and shared. Ross's laugh stood out because it matched the route's unsettling tone, and as a short standalone sound it has since been reused in memes and edits whenever a creator wants a distinctly gleeful, villainous reaction.