Place it at the start of a boss reveal, after an Enemy Spotted cue and before the action begins, then follow it with an Inception Horn sting to raise the stakes for the fight ahead.
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This five-second clip is a playful remix of Bowser's jingle from Super Mario World, with bright chiptune notes and a mischievous villain theme. It feels cheeky and dramatic, like a cartoon boss making a grand entrance. The remix comes from a secret track hidden in the Sound Journal For Lovers ROM.