Place it at the end of a fail sequence, right after the losing play resolves. Lead in with the Metal Gear Alert Sound when the mistake gets spotted, then use this clip as the closer, and follow a lighter beat with the Ba Dum Tss Drum Sting for a less serious cut.
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This is a short 2.5 second burst of Shao Kahn laughing, a deep theatrical taunt delivered in his boss voice. It feels smug, cruel and gleefully mocking, turning a win into pure domination. The clip comes from Mortal Kombat 3, the 1995 fighting game where Shao Kahn serves as the final boss.