Place it just before a reveal, after a Dun Dun Dun build-up, so the moment peaks right before the payoff, then follow it with a Vine Boom to punctuate the scene and reset it for the next joke.
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The clip is a single sustained 'S' hiss lasting about 2.3 seconds, a breathy sound on its own, with no melody or beat behind it. It feels sharp and tense, like a warning whispered just out of sight, and it can also read as playful when it cuts through a quiet moment. That simple shape makes it easy to recognise at once.