Place it near the end of a teasing scene, just before the reaction shot, then follow it with an Anime Ahh Sound Effect for a bigger reaction, or an Awkward Moment Sound Effect when the joke should linger instead of escalating.
Об этом звуке
This is a short voice clip of a young anime girl saying please stop in Japanese, stretched out in a high, soft and embarrassed tone that sounds more pleading than angry. It feels funny because the gentle delivery makes a desperate request sound cute and over the top, like someone overwhelmed by teasing. The line comes from the Japanese phrase yamete kudasai, meaning please stop.