The sound comes from Super Mario Bros, Nintendo's landmark platform game first released in 1985, where it plays whenever Mario loses a life after touching an enemy or falling into a pit. As the game became one of the most played and referenced titles in history, its short death jingle spread far beyond the console through gameplay videos, remixes and meme edits. Today it works as a universal shorthand for failure, instantly understood even by viewers who have never played the original game.