This sound comes from Among Us, the multiplayer social deduction game that surged in popularity in 2020. It is associated with the card swipe task, one of the game's most recognizable mini games because players often failed it repeatedly. As clips and streams spread online, the deny beep became a shorthand for failure, rejection and user error. It still appears in short memes, gaming compilations and reaction edits because the meaning is understood almost instantly, similar to a Windows XP Error style cue.